The Waals™ RNA Pull-down Kit is a specialty kit specifically designed for RNA pull-down experiments. It works by connecting synthesized RNA with biotin-labeled RNA, then attaching it to streptavidin magnetic beads, followed by incubation with protein samples and subsequent elution.
Product Features:
Preservation of Natural RNA Structure: Through its uniquely designed process, this kit ensures the maximum preservation of the natural structure of RNA during the experimental process.
High Efficiency: Utilizing the high affinity between biotin and streptavidin, the kit greatly enhances the efficiency of RNA purification.
User-friendly: The kit includes all necessary reagents for RNA pull-down experiments along with detailed instructions, making the experimental process straightforward and easy-to-follow.
Patented: The production and application of this kit have been granted a national invention patent, reflecting its advanced technology and practical value.
Application Fields:
The Waals™ RNA Pull-down Kit is primarily used in the study of RNA-protein interactions. By identifying specific protein partners of certain RNAs, it aids in unveiling the biological functions of RNA and its regulatory mechanisms.
Instructions for Use:
When conducting experiments using the Waals™ RNA Pull-down Kit, start by connecting the synthesized RNA with biotin-labeled RNA, then attach it to streptavidin magnetic beads and purify. Next, incubate the purified RNA with protein samples to form complexes with the target protein. Finally, perform the elution step. The resultant eluate contains proteins that specifically bind to the RNA and can be used for subsequent protein analysis.
Precautions:
For optimal results, it is suggested to strictly follow the guidelines provided during the experimental process.
The experiment should be carried out under RNase-free conditions to prevent RNA degradation.